06 January 2025
Sunaryo S.Kom
PHARMACY PUBLIC RELATIONS USU – The Faculty of Pharmacy, Universitas Sumatera Utara, conducted the Doctoral Promotion Examination (Open Defense) for the Doctoral Program in Pharmaceutical Sciences on Thursday, December 19, 2024, at 2:00 PM WIB in the IMT-GT Room, 2nd Floor, BPA Building, USU.
The doctoral candidate was Bunga Rimta Barus, with the dissertation titled:
"Antibacterial Activity of Red Ginger Leaves (Zingiber officinale Rosc. Var. Rubrum) and Gel Formulation of Its Active Fraction as an Anti-Inflammatory Agent Against Infected Excision Wounds"
and the scientific article titled:
"Therapeutic Effect of Red Ginger Leaf Ethyl Acetate Fraction Gel on Staphylococcus aureus-Infected Excision Wounds on Rat: A Preclinical Study"
Defense Committee Chair:
Prof. Dr. Muryanto Amin, S.Sos., M.Si. (Rector, Universitas Sumatera Utara)
Supervisory Committee:
Prof. Dr. Masfria, M.S., Apt. (Promoter)
Dr. Aminah Dalimunthe, S.Si., M.Si., Apt. (Co-Promoter)
Dr. Sovia Lenny, S.Si., M.Si. (Co-Promoter)
External Examiners:
Prof. Dr. rer. nat. Effendy De Lux Putra, S.U., Apt.
Dr. Panal Sitorus, M.Si., Apt.
Prof. Dr. Herbert Sipahutar, M.S., M.Si.
Prof. Dr. Martina Restuati, M.Si.
Red ginger leaves have been traditionally used for wound healing due to their flavonoid content, including quercetin, luteolin, and resveratrol. This research aimed to evaluate the antibacterial activity of ethanol extract and the active fraction of red ginger leaves, identify their compounds, and develop an ethyl acetate fraction gel formulation for excision wounds infected with Staphylococcus aureus, assessing anti-inflammatory effects using parameters such as wound area, healing percentage, histopathology, and TNF-α expression.
Antibacterial activity was tested in vitro using the diffusion method. The ethyl acetate fraction was isolated using column chromatography, and active compounds were analyzed with UV-Vis spectrophotometry, FTIR, LC-MS/MS, and NMR. The active fraction was formulated into gels at concentrations of 1%, 3%, and 5%. Antibacterial and anti-inflammatory evaluations were conducted with voltaren as the positive control and gel base as the negative control. Parameters included inhibition zone diameter, epidermal thickness, angiogenesis count, and TNF-α levels.
The study found that the 5% ethyl acetate fraction gel was the most effective, showing an inhibition zone diameter of 15.07 ± 0.15 mm, epidermal thickness of 45,423.84 ± 7,235.03 μm, angiogenesis count of 26.33 ± 0.31, and TNF-α levels of 81.3 ± 25.05 pg/mL. The gel formulation met all evaluation standards and proved effective in bacterial inhibition and wound healing in infected rats.
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