25 June 2024
Sunaryo S.Kom
USU PHARMACY PUBLIC RELATIONS – Faculty of Pharmacy, University of North Sumatra has carried out the Doctoral Promotion Examination (Open Session) for the Doctor of Pharmaceutical Sciences Program on Monday, June 24 2024 at 14.00 WIB – finished, in the IMT-GT Room, Fl. 2 USU BPA Building.
Promovenda at this open session was Dadang Irfan Husori, with a dissertation, "Study of the Gastroprotective Effect of Ethanol Extract of Breadfruit Leaves (Artocarpus altilis (Parkinson) Fosberg) on Rat Gastric Ulcers Induced by Pyloric Ligation and Mucosal Damage"; and the scientific article, "Toxicological Safety Evaluation of Ethanol Extract of Artocarpus altilis Leaves in Wistar Rats (Rattus norvegicus)"
Chair of the session is the Chancellor of the University of North Sumatra, Prof. Dr. Muryanto Amin, S.Sos., M.Sc. With Supervisory Commission: Prof. Dr. Urip Harahap, Apt. as Promoter; Prof. Dr. Syafruddin Ilyas, M. Biomed. as Co Promoter; and Dr. Aminah Dalimunthe, S.Sc., M.Sc., Apt. as Co Promoter. Meanwhile, External Commission Examiner: Prof. Dr. Poppy Anjelisa Z. Hasibuan, M.Sc., Apt.; Prof. Dr. Ginda Haro, M. Sc., Apt.; Prof. Dr. Agung Endro Nugroho, M.Si., Apt.; and Prof. Madya Dr. Satirah Zainalabidin.
Brief Description of the Promovenda Dissertation:
Breadfruit (Artocarpus altilis) is a plant that is widespread in Indonesia which has the potential to anti-gastric ulcers. Breadfruit contains flavonoids and has anti-inflammatory, antibacterial, cytoprotective and wound healing effects. The aim of this study was to assess the mechanism of gastroprotective activity of breadfruit leaf ethanol extract (EEDS) and assess its safety. In silico research was carried out using Prediction of Activity spectra for Substance, SwissADME, pk-CSM-Tools, Pro-Tox II Tools and AutoDock Vina using 19 breadfruit leaf flavonoid compounds and molecular docking on the histamine-2 receptor (PDB Code: 6H7J) and pump proton (5YLU). Gastroprotective in vivo research using male mice as a model of gastric ulcer, pyloric ligation and mucosal damage. Each model consists of 6 groups (@5 mice): normal, carrier, positive (omeprazole 25 mg/kg BW/sucralfate 360 mg/kg BW), EEDS (50, 100, 200 and 400 mg/kg BW). Rats were treated for 7 days, fasted and then induced by pylorus ligation or administration of 2 ml/200 mg ethanol. Animals were sacrificed for macroscopic, microscopic, secretion and biochemical observations. Acute toxicology testing for 14 days used 20 female mice, namely the control group, EEDS 500, 2,000 and 5,000 mg/kg BW. Subchronic toxicity was carried out on male and female mice for 90 days, namely the control group, EEDS 125, 250, 500, and satellite 500 mg/kg BW. In silico results show that breadfruit leaf flavonoids have biological activities that are relevant to gastroprotective, namely anti-inflammatory, antioxidant, lipid peroxidase inhibitor, anti-ulcer, membrane integrity agonist, cytoprotector, membrane permeability inhibitor, mucous membrane protector, histamine release inhibitor and anti-Helicobacter pylori. Pharmacokinetics and physicochemistry are similar to drug compounds. Breadfruit leaf flavonoids are linked to proton pumps and H-2 receptors. EEDS in vitro has antioxidant activity. In vivo, EEDS reduces the number of ulcers, ulcer area and ulcer index, increases the percentage of ulcer inhibition, increases mucus secretion and gastric pH, reduces gastric fluid volume and total gastric fluid acidity. EEDS also increases SOD and GSH levels, GPx and PGE2 expression, decreases MDA and COX-2 expression. The optimal dose of EEDS as gastroprotective is 400 mg/kg BW. The LD50 of EEDS is >5,000 mg/kg BW and subchronic toxicity is safe for long-term use.
EEDS can be concluded to have gastroprotective activity by inhibiting gastric acid production through binding to proton pumps and H-2 receptors, maintaining the integrity of mucous cell membranes in mice with induced gastric ulcers. EEDS is practically safe and can be used for the long term.
CONGRATULATIONS & SUCCESS to Dr. Dadang Irfan Husori, S.Si., M.Sc., Apt. for achieving a Doctorate degree.
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